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The firassay of ferrous gluconate and iron (II) acetate was conducted to compare their efficacy against ferrous bisglycinate and ferric pyrophosphate. This study highlights the significance of natural factors, particularly potassium citrate, in enhancing iron absorption. The interactions of these compounds with potassium citrate were analyzed, revealing how natural factors play a critical role in optimizing iron supplementation. In conclusion, understanding the influence of natural factors like potassium citrate is essential for improving the bioavailability of iron sources such as ferrous gluconate, ferro (II) acetate, and their counterparts.